Fujioka-Kobayashi M, Schaler B, Shirakata Y, Nakamura T, Noguchi K, Zhang Y, Miron RJ. Int J Oral Maxillofac Implants. 2017; 32(4):e221-e230.
The bone-inducing properties of Jason® membrane and mucoderm® in combination with recombinant human bone morphogenetic protein (rhBMP)-2 and rhBMP-9 on osteoblast behavior were analyzed.
Both membranes were coated with rhBMP-2 or rhBMP-9, respectively. The adsorption and release abilities were investigated via ELISA assay. Cell adhesion, proliferation, and osteoblast differentiation were assessed by MTS assay and real-time PCR by using murine bone stromal ST2 cells.
Results / Conclusion:
Both rhBMP-2 and rhBMP-9 adsorbed to collagen membranes and were gradually released up to 10 days. Both rhBMPs were shown not to affect cell proliferation, collagen membranes combined with rhBMP-9 significantly increased ALP activity and ALP mRNA levels. rhBMP-9 increased osteocalcin mRNA levels and alizarin red staining. Both porcine-derived collagen membranes combined with rhBMP-9 accelerated the osteopromotive potential of ST2 cells.