Stähli A, Miron RJ, Bosshardt DD, Sculean A, Gruber R. J Periodontol. 2016; 87(5):583-90.
Enamel matrix derivative (EMD) and collagen membranes (CMs) are simultaneously applied in regenerative periodontal surgery. Aim of this study is to evaluate the ability of two CMs and a collagen matrix to adsorb the activity intrinsic to EMD that provokes transforming growth factor (TGF)-β signaling in oral fibroblasts.
Collagen products were exposed to EMD or recombinant TGF-β1. Oral fibroblasts were either seeded directly onto collagen products or were incubated with the respective supernatant. Expression of TGF-β target genes interleukin (IL)-11 and proteoglycan 4 (PRG4) were evaluated by PCR.
Results / Conclusion:
EMD or TGF-β1 provoked a significant increase of IL-11 and PRG4 expression when seeded onto collagen products and when incubated with the respective supernatant. The in vitro findings suggest that collagen products adsorb a TGF-β receptor I kinase-dependent activity of EMD and make it available for potential target cells.