Biofunctionalization of porcine-derived collagen matrices with platelet rich fibrin: influence on angiogenesis in vitro and in vivo

Blatt S, Burkhardt V, Kämmerer PW, Pabst AM, Sagheb K, Heller M, Al-Nawas B, Schiegnitz E. Clin Oral Investig. 2020. [Epub ahead of print]

A pre-clinical study on the angiogenesis of botiss collagen membranes

Evaluation of the influence of platelet-rich fibrin (PRF) on the angiogenesis of different collagen-derived membranes in vitro and in vivo.
Three different collagen-derived membranes (mucoderm®, Jason® membrane, and collprotect® membrane) were combined with PRF in a plotting process. Growth factor release (VEGF, TGF-β) was measured in vitro after 1, 4 and 7 days in comparison and to PRF alone. The angiogenic potential was analyzed in vivo after 24 h and verified with immunohistochemical staining for CD105 and αSMA in an in ovo yolk sac (YSM) and chorion allantois membrane (CAM) model.
Highest growth factor release was seen after 24 h for all three activated membranes in comparison to the native membranes and PRF. All activated membranes revealed a significantly increased angiogenic potential in vivo after 24 h.
PRF improved the angiogenesis of collagen-derived membranes in vitro and in vivo.

Sebastian Blatt, Eik Schiegnitz

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