The goal of the study was to evaluate the effect of four differently processed bovine bone substitute materials on the viability, metabolic activity of human osteoblasts as well as on the expression of genes crucial for bone mineralization. In addition, the effect of iPRF on all parameters was assessed.
Human osteoblasts were cultured with one of the following bone grafting materials, either in the presence or absence of iPRF: cerabone®, Bio-Oss®, Creos Xenogain®, MinerOss® X. Cell viability and metabolic activity were assessed using immunofluorescence microscopy and MTT assay respectively. Expression of bone gene markers alkaline phosphatase, bone morphogenetic protein 2, and osteonectin was examined using real time PCR. Parameters were evaluated at 3, 7 and 10 days following incubation.
Highest cell viability in the cerabone® group
Addition of iPRF increased cell viability in all groups with the highest values for cerabone® at 3 and 7 days
Highest cellular metabolic activity in the cerabone® + iPRF group among the evaluated bone grafting materials
No overall significant differences in gene marker expression among the evaluated bone grafting materials
Significantly increased viability of human osteoblasts by cerabone®
Higher osteoblastic biological activities for cerabone® compared to non-sintered bovine bone grafts
Addition of iPRF resulted in significantly increased biological activities
Results suggest that high temperature treatment of cerabone® is beneficial for osteoblast activation
shows optimal biocompatibility for bone forming cells
stimulates osteoblast activation and proliferation essential for new bone formation
promotes expression of osteoblastic genes important for bone mineralization